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Request Your Free ZipScript ™ Sample

ZipScript is a new
One-Step RT-qPCR mix
from Enzymatics Inc.

SGS - inspection, verification, testing; certifica
British Society for Immunology
British Society for Histocompatibility & Immunogen
 
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Immunocolumns - Cedarlane
How do your immunocolumns work?
What is the expected cell yield and purity when used properly?
Do I have to use your Lympholyte® cell separation media when isolating my cells before loading them onto the column?
Can I load more cells than what is specified?
For the Human immunocolumns, can I run the column flow rate faster or slower than 6-8 drops per minute?
Will the columns remove macrophages and monocytes in addition to certain lymphocytes?
Can I reuse your immunocolumns?
What is the shelf life of these kits when stored at 4°C?
What is the difference between your "newly improved" columns and the original columns?
Can I freeze your immunocolumns to extend the shelf life?
Lympholyte - Cedarlane
Is there an expiry date for your Lympholyte® products?
I have Lympholyte® that is more than 5 years old. Is it OK to use?
You have several different Lympholyte® formulations. How do I know which one to use?
Can I use Lympholyte®-M with peripheral blood samples, in addition to cells isolated from mouse tissue?
Why do I have to use a serum-free media when using Lympholyte® for cell isolation?
Can I isolate granulocytes using Lympholyte®?
Quantidex™ DNA Assay - Asuragen
What is the Quantidex DNA assay?
How does the assay work?
How is the QFI™ score calculated?
How is the presence of inhibitors decided?
What is included in the kit? Do I need to buy any other reagents?
Which Instrument is needed?
How long does it take to run the assay?
Does this assay replace quantitation by spectrophotometers or Qubit?
Why is the Quantidex DNA Assay better than Qubit or Spectorphotometry at predicting true DNA quality and downstream success of NGS?
Why is the Quantidex DNA Assay better than other qPCR based assays at predicting true DNA quality and downstream success of NGS??
How do I analyse the data produced by the Quantidex DNA Assay?
You are amplifying an 82 bp amplicon. My average library size is x bp. How is your QFI™ score applicable to my library size?
Rabbit Complement - Cedarlane
You have several different complement formulations available. How do I know which type to use?
What is special about the Low-Tox formulations?
What dilutions should I normally use?
What is the shelf life of your complement?
Can I use expired complement for my assay?
Can I re-use my reconstituted complement?
The isotype of my primary antibody will not fix complement. Can I use a secondary antibody that fixes complement to deplete cells labeled with this primary antibody?
How do I sterilize complement for assays requiring sterile product?
Is it better to use frozen or lyophilized complement?
Guinea Pig Complement - Cedarlane
You have several different complement formulations available. How do I know which type to use?
What is special about the Low-Tox formulations?
What dilutions should I normally use?
What is the shelf life of your complement?
Can I use expired complement for my assay?
Can I re-use my reconstituted complement?
The isotype of my primary antibody will not fix complement. Can I use a secondary antibody that fixes complement to deplete cells labeled with this primary antibody?
How do I sterilize complement for assays requiring sterile product?
Is it better to use frozen or lyophilized complement?
Human Complement - Cedarlane
You have several different complement formulations available. How do I know which type to use?
What is special about the Low-Tox formulations?
What dilutions should I normally use?
What is the shelf life of your complement?
Can I use expired complement for my assay?
Can I re-use my reconstituted complement?
The isotype of my primary antibody will not fix complement. Can I use a secondary antibody that fixes complement to deplete cells labeled with this primary antibody?
How do I sterilize complement for assays requiring sterile product?
Is it better to use frozen or lyophilized complement?
MolYsis Kits - Molzym
Why has human DNA to be removed before PCR assaying of bacteria and fungi?
How is human DNA removed during blood processing?
Do I have to change my validated DNA purification system?
Can MolYsis™ be used with automated DNA purification systems?
What kind of consumables are to be used?
What kind of samples can be processed with MolYsis™?
What is the sample volume that can be processed?
What is the sensitivity of detection of bacteria and fungi in blood?
Can blood cultures be used for isolation of microbial DNA?
Automated Pathogen DNA Isolation - Molzym
What are the specifications of the instrument, SelectNA™?
What are the clinical samples that can be processed by the SelectNA™?
How many extractions can be run by the SelectNA™?
What is the volume of sample processed?
What is the processing time of pathogen DNA isolation?
What are the components delivered with the 'Blood Pathogen' kit for the SelectNA™ instrument?
How does the DNA isolation by the SelectNA™ work?
How is the DNA-free state of all reagents and consumables of the kit guaranteed?
Genomic DNA Extraction Kits - Molzym
What are the yields of genomic DNA from different samples?
What are the maximum amounts of samples that can be processed?
Are highly viscous solutions a problem?
At which points can the DNA isolation procedure be interrupted?
Why is genomic DNA eluted with heated buffer EB?
Does elution buffer EB interfere with downstream applications?
Can buffers other than EB used for elution of nucleic acids?
PCR Master Mixes - Molzym
Why is universal 16S rDNA PCR a promissing option of bacteria detection?
What is the sensitivity of detection?
What reagents can bear contaminations by bacterial DNA?
How can bacteria be identified when detected by 16S rDNA PCR?
Can MolTaq 16S and Mastermix 16S products be used for Real-Time PCR?
Does Molzym manufacture customized mastermixes?
For what application do I need the primers?
Do the primers differentiate between Gram-positive and Gram-negative bacteria?
What to do with overlapping sequences?
Thermostable DNA Polymerases - Molzym
Can MolTaq be used for hotstart amplification?
What is the effect of the PCR enhancer delivered?
Can MolTaq be used for real time PCR?
What is the function of the red dye in MolTaqRed and MolTaqRed Mastermix?
What is the source of MolTaq products?
Mouse Feeder Cells - GlobalStem
What are feeder cells?
Why are mouse embryonic fibroblast (MEFS) used when culturing ES cells?
At what density should I plate my MEF feeder cells?
Can I use your MEFs for human ES cells?
How many passages can MEFs be expanded?
Can I use gelatin when plating MEFs?
How soon after plating my MEF can I plate cells?
Can I use gelatin when plating MEF?
"How soon after plating my MEF can I plate cells? "
"What type of tests do you perform to ensure consistence between different lots of cells? "
"What are the mouse pathogens that you screen for?"
"What method does MTI-GlobalStem use for screening mouse pathogens? "
Human Feeder Cells - Globalstem
What are NuFF cells?
"What medium should I use to grow my mitomycin-C treated NuFF? "
"How many times can NuFF cells be expanded? "
What medium should I use to grow MTI-GlobalStem Mitomycin-C treated NuFF?
How many times can the NuFF be expanded?
What type of test do you perform to ensure consistence between different lots?
Stem Cell Media & Foetal Bovine Serum - Globalstem
What is the composition of the PluriQ™ hESC and mESC Freeze Media?
Assays & References - Globalstem
Are 2102Ep EC cells sensitive to media?
"What tissue culture plate coating should I use to grow the cells? "
DNA Library Construction Kits - Enzymatics
Under what circumstances would I use SPARK™ DNA Sample Prep Kit for Illumina®
How much DNA is required for SPARK™ Kits?
How long is the SPARK™ DNA Sample Prep Kit for Illumina® stable at room temperature?
I've opened a foil pouch. How quickly must I use the remaining kit components?
I left a reaction tube outside the foil pouch. Can I still use it?
Can the lyophilized reaction mixes be transferred to a different reaction vessel?
I am unable to incubate the 1.5 mL tubes at the temperatures indicated in the protocol. What should I do?
Does SPARK™ DNA Sample Prep Kit for Illumina® come in any other formats than 1.5 mL tubes?
Can Size Selection successfully be performed after End Repair instead of after Adaptor Ligation?
Can I use QIAGEN® MinElute® Reaction Cleanup Kit columns to purify after each reaction rather than using AMPure® XP beads?
How do I calculate the amount of A-Tailed DNA (X) that I need for Adaptor Ligation?
How do I calculate the elution volume (Z) for the post-adaptor ligation purification?
How do I perform the second bead based purification that is recommended after ligation when not employing size selection?
Do you have any recommendations for a bead based size selection protocol?
What is recommended for with-bead purification library construction?
RNA Enzymes - Enzymatics
What are the advantages of EnzScript™ M-MLV Reverse Transcriptase RNase H- (P7600) versus wild type M-MLV Reverse Transcriptase (P7040)?
What types of priming are compatible with EnzScript™ M-MLV RT RNase H-?
What is the optimal reaction temperature for EnzScript™ M-MLV RT RNase H-?
What PCR enzymes can be used following first-strand synthesis?
What is the suggested protocol for generating long, full-length cDNA transcripts (> 5 kB)?
Ultrapure Polymerases - Enzymatics
How stable is Phoenix Hot Start Taq when incubated in a PCR reaction mix at room temperature?
How can PCR cycling conditions be optimized for Phoenix Hot Start Taq?
Can Phoenix Hot Start Taq utilize cDNA as template for PCR?
Is Phoenix Hot Start Taq capable of multiplex PCR?
How can I optimize Mg2+ conditions for a specific amplicon when using Phoenix Hot Start Taq and the supplied reaction buffer?
When should I use Phoenix Hot Start Taq GC reaction Buffer?
What is the amplification length limit of Phoenix Hot Start Taq?
What is the fidelity/error rate of Phoenix Hot Start Taq?
Why is the Phoenix Hot Start Taq sometimes cloudy upon removing from -20°C storage?
How can yield for long targets be increased when using Phoenix Hot Start Taq?
How is VeraSeq 2.0 High-Fidelity DNA Polymerase different from the standard recombinant Taq-B DNA Polymerase?
How can I optimize Mg2+ conditions for a specific amplicon when using VeraSeq 2.0 High-Fidelity DNA Polymerase and the supplied reaction buffers?
When should I use 5X VeraSeq GC Buffer?
What is the amplification length limit of VeraSeq 2.0 High-Fidelity DNA Polymerase?
What is the fidelity/error rate of VeraSeq 2.0 High-Fidelity DNA Polymerase?
Why is VeraSeq 2.0 High-Fidelity DNA Polymerase sometimes cloudy upon removing from -20°C storage?
How can yield for targets be increased when using VeraSeq 2.0 High-Fidelity DNA Polymerase?
Will VeraSeq 2.0 High-Fidelity DNA Polymerase incorporate dUTP?
Do the DNA fragments generated by VeraSeq 2.0 High-Fidelity DNA Polymerase have a single-base 3´ overhang?
Is VeraSeq 2.0 High-Fidelity DNA Polymerase available as a hot start enzyme?
How long can reaction components incubate with VeraSeq 2.0 High-Fidelity DNA Polymerase at room temperature prior to PCR cycling?
What denaturation temperature should be used in the cycling conditions?
What annealing temperature should be used in the cycling conditions?
What is the stability of VeraSeq 2.0 High-Fidelity DNA Polymerase at room temperature?
How is VeraSeq ULtra DNA Polymerase different from the standard recombinant Taq-B DNA Polymerase?
How can I optimize Mg2+ conditions for a specific amplicon when using VeraSeq ULtra DNA Polymerase and the supplied reaction buffers?
What is the amplification length limit of VeraSeq ULtra DNA Polymerase?
What is the fidelity/error rate of VeraSeq ULtra DNA Polymerase?
Why is VeraSeq ULtra DNA Polymerase sometimes cloudy upon removing from -20°C storage?
How can yield for targets be increased when using VeraSeq ULtra DNA Polymerase?
Will VeraSeq ULtra DNA Polymerase incorporate dUTP?
Do the DNA fragments generated by VeraSeq ULtra DNA Polymerase have a single-base 3°C overhang?
Is VeraSeq ULtra DNA Polymerase available as a hot start enzyme?
How long can reaction components incubate with VeraSeq ULtra DNA Polymerase at room temperature prior to PCR cycling?
What is the stability of VeraSeq ULtra DNA Polymerase at room temperature?
Acella Competent Cells - Edge
How are cells made competent?
How do I calculate the transformation efficiency?
Chemically Competent Cells - Edge
How are cells made competent?
How do I calculate the transformation efficiency?
Electrocompetent Cells - Edge
How are cells made competent?
How do I calculate the transformation efficiency?

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