The involvement of epigenetic inheritance in development is a large area of study. DNA methylation is essential for proper mammalian embryonic and postnatal development and aberrant DNA methylation has been implicated in a number of diseases. High throughput sequencing has allowed for genome-wide comparison of methylation states in disease and normal samples, opening the door for large-scale studies in many fields such as cancer, development and infectious disease.

This technique gives a picture of the protein-DNA interactions involved in gene regulation or chromatin organization. Determining how proteins interact with DNA to regulate gene expression is essential for fully understanding many biological processes and disease states.

Sequencing millions of ChIP-enriched DNA fragments using next generation sequencing technology enables cost-effective and precise analysis of the binding sites of transcription factors, replication and transcriptional machinery, structural proteins such as histones, as well as the impact of protein modifications on genome occupancy

Hybridization-based methods are the most common method for genome-wide analysis of DNA-binding proteins (ChIP-chip), which combines chromatin immunoprecipitation with DNA microarrays.  The major limitation of this hybridization-based method is that microarrays are restricted to a fixed number of probes, thereby introducing bias.

Pricing is dependent on organism and desired project design. For further information please contact our Molecular Biology Product Manager, Catherine Mair (email: cmair@vhbio.com or tel: +44 (0)191 495 8211).