AmplideX® technology has revolutionised how repeat expansions within the FMR1, C9orf72, and TOMM40 genes can be analysed, significantly expediting time-to-result and reducing the reliance on cumbersome Southern blot analysis.
The AmplideX® PCR/CE DMPK Kit extends this groundbreaking approach to the DMPK gene, which has long challenged laboratory analysis due to the presence of very large expansions (over 1000 repeats) and a high degree of mosaicism.
The AmplideX® DMPK kit, which is for use on widely established CE systems, overcomes these challenges and enables the sensitive detection and sizing of these DMPK repeats all within a single laboratory shift – without the need for Southern blot confirmation.
SIMPLE WORKFLOW FOR DMPK REPEAT EXANSIONS
Shares a common workflow with existing AmplideX® products, like FMR1.
Go from DNA to data in a single laboratory shift.
Runs on established platforms and eliminates need for Southern blot.
SIMPLIFIED ANALYSIS OF THE DMPK GENE
Blood, Buccal, CVS.
20-80ng DNA (in 2µl)
Applied Biosystems 3130 / 3500 / 3730 Genetic Analyzers
RESOLVE ZYGOSITY AND DETECT MOSAICISM
CLEARLY DISCERN BETWEEN HOMO- AND HETEROZYGOUS ALLELES
ACCURATELY SIZE EXPANSIONS UP TO 200 REPEATS
REDUCES THE NUMBER OF EXPANDED SAMPLES REQUIRING FURTHER RESOLUTION
ELIMINATES NEED FOR SOUTHERN BLOT
SIZE BEYOND 200 REPEATS WITH AGE WORKFLOW