Custom Target Enrichment Panels

VH Bio supplies NGS Custom Target Enrichment Panels from Celemics, which produce higher coverage and in-depth sequencing data for your specific genomic loci of interest, therefore increasing the sensitivity and efficiency of variant discovery.

Celemics Target Enrichment Panels use hybridisation-based capture technology to enable the core process of Target Sequencing, which is the separation and analysis of specific regional sequences of entire genomes. It is able to accurately analyse all types of mutations, such as SNV, InDel CNV, and Rearrangement.

Celemics’ Target Enrichment Panel utilises their intrinsic probe design, rebalancing, and molecular barcode technologies to efficiently analyse not only hard-to-capture areas such as GC-rich and homologous regions but also samples originating from circulating tumour DNA and FFPE with extremely small amounts of damaged DNA and RNA as well.

Features and Benefits of Celemics’ NGS Target Enrichment Panel Technology:

Industry-leading capture performance:

• Industry-leading on-target ratio, coverage, and uniformity

High performance, even in difficult to analyse areas:

• While other companies purposely mask hard-to-capture areas such as GC-rich and homologous regions, which makes important mutation analysis difficult, Celemics’ proprietary technology guarantees great capture performance, even in these areas.

 Generate libraries from low input intact or highly fragmented FFPE material

• Accurate analysis possible with inputs as low as 20ng
• High performance even with damaged samples originating from FFPE or circulating tumour DNA (ctDNA)

Cost-effective pre-capture pooling workflow available:

• Capable of analysing several sample libraries through one capture by attaching sample indexes beforehand when creating libraries, significantly decreasing experimental hands-on time and cost.

Provision of molecular barcode kits and bioinformatics:

• Provision of molecular barcode kits specifically developed to detect mutations in samples with low amounts of material, such as ctDNA, and bioinformatics algorithm that eliminates, duplicates and generates consensus sequences

Gene add-on possible to pre-existing panels:

• Decrease novel panel development time and cost through the addition of new clinically significant genes or general panel expansion to already existing client panels

Provision of kits for sample cross-contamination prevention:

• Provision of kits that monitor and prevent sample cross-contamination using artificial vector sequences

 Support of enzymatic DNA fragmentation:

• Workflows support both ultrasonic-based mechanical shearing and enzymatic fragmentation

Bioinformatics software:

• Provision of bioinformatics software that processes raw NGS data (FASTQ) to identify characteristics such as mutation type, VAF, and pathogenicity

Pre-performance validation

• Celemics is the only company to guarantee performance and provide detailed QC information to clients before shipment of every customised panel
• Reduces the possibility of experimental failure
• Proprietary probe rebalancing technology to ensures the highest uniformity and coverage

Celemics’ Target Enrichment Panel is compatible with all currently available NGS equipment, such as Illumina, Thermo Fisher Scientific, Pacific Bioscience, and MGI, and provides bioinformatics software for data analysis.

Table 1. Panel manufacturing process

Table 2. Pilot test and panel rebalancing

Table 3. Other company’s targeted custom panel IGV data for homologous regions

Table 4. Sequencing performance of Celemics panel for GC-rich region

Contact VH Bio for more info or to place an order.