LABScreen™ Single Antigen Supplement

Extended HLA allele coverage facilitating identification of HLA-specific antibodies and epitope-level patterns of antibody reactivity.

Allelic-level HLA typing of donors and recipients is now commonplace across solid organ as well as stem cell transplantation.  This provides opportunity to define HLA antibodies to a greater degree, aiding in more nuanced transplant decision making particularly amongst highly sensitised patients with reduced chance of transplant.  The increased breadth of allele coverage provided by the LABScreen™ Single Antigen Supplement assay increases the number of patients who can benefit from allelic and epitope-level assessment of donor-recipient immunological compatibility with the aim of effecting better clinical outcomes.

The LABScreen™ Single Antigen Supplement assay builds upon the widely used LABScreen™ Single Antigen assay.  HLA antibody detection coverage is expanded with the addition of 54 HLA Class I and 24 HLA Class II Single Antigen beads.  

• Paired with the standard panel, allele coverage increases to 151 Class I and 119 Class II common and well-documented (CWD) alleles, providing better representation of diverse communities.
• Complements high-resolution donor HLA typing when identifying donor specific antibodies in both pre-transplant crossmatching (virtual or wet) and post-transplant scenarios.
• Increased breadth of epitope coverage facilitates and increases confidence in epitope-based analysis.

Additional HLA allele coverage

Workflow summary:

• Familiar LABScreen laboratory workflow.
• Acquisition and reading by the LABScan100™ (Luminex 200) or LABScan3D™ instrument.
• Users of the LABScan3D™ instrument can benefit from extended multiplexing capability using the LABScreen Single Antigen ExPlex panels for single-well set-up, providing ease and flexibility.
• Analysis in HLA Fusion™ software (combined or separately) with inbuilt HLA Matchmaker, Epitope Panel and Amino Acid utilities for epitope-based analysis.

Growing numbers of sensitised patients are requiring increasingly complex assessment of donor-recipient immunological compatibility as well as post-transplant monitoring.  The LABScreen™ Single Antigen Supplement assay can be a simple yet effective part of your tool kit to decipher patterns of antibody reactivity with confidence, contributing to improved outcomes for your patients.

Find out more

• Webinar: Refining HLA Antibody assignment using the LABScreen Single Antigen ExPlex Bead Panel. Speakers: Rob Liwski MD, PhD, FRCPC, A(ACHI) & Sandra Tafulo, PhD

• https://www.vhbio.com/antibody-detection-identification-analysis/
• https://www.vhbio.com/improved-hla-antibody-detection-transplantation-virtual-crossmatching/
• PubSummary: Incidence and impact of allele-specific anti-HLA antibodies and high resolution HLA genotyping on assessing immunologic compatibility