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Low Toxicity Rabbit complement

For over 50 years, Cedarlane has been producing complement and has grown to become a worldwide leader in supplying reagent complement. Special processing techniques are used to yield complement with high activity and low background cytotoxicity. We offer complement from a number of species and in multiple formats to suit a variety of applications. Cedarlane […]

For over 50 years, Cedarlane has been producing complement and has grown to become a worldwide leader in supplying reagent complement. Special processing techniques are used to yield complement with high activity and low background cytotoxicity. We offer complement from a number of species and in multiple formats to suit a variety of applications. Cedarlane offers bulk quantities, reserved lot evaluation, and custom formats and sizes.
• Low-Tox®-M Rabbit Complement for use with Mouse Cells
Rabbit serum provides the most potent source of complement for cytotoxicity techniques utilizing antisera to mouse lymphocytes. However, rabbit serum is very toxic to mouse lymphocytes, particularly thymocytes. This toxicity can be reduced significantly by appropriate absorptions. Each lot is thoroughly tested for low toxicity and high activity, and can be used without further processing.
Main Application – Cytotoxicity assays using MOUSE lymphocytes.
• Low-Tox®-H Rabbit Complement for use with Human B-Lymphocytes
Low-Tox®-H Rabbit Complement is a normal rabbit serum that has been processed for low toxicity to human peripheral blood B-lymphocytes. It is intended for use as a source of complement for HLA-DR tissue typing and all standard lymphocytotoxicity assays, particularly those that utilize cells sensitive to lysis by pooled normal rabbit serum. The cells are pre-incubated with the appropriate antiserum, then incubated with the complement source. After the appropriate time, the degree of cytotoxicity is scored. The conditions of testing will vary depending on the cell type and method of scoring. The standard microlymphocytotoxicity techniques are published in the NIAID Manual of Tissue Typing Techniques. It is important that appropriate complement controls be run in all assays as background toxicity will vary between systems.
Main Application – HLA-DR tissue typing
• Low-Tox®-R Rabbit Complement for use with Rat Cells
Cedarlane Low-Tox®-R Complement is derived from a highly potent mammalian source of complement, and is adsorbed with agarose and sheep erythrocytes to reduce nonspecific cytotoxicity to rat lymphocytes. This process provides an excellent source of complement for cytotoxicity techniques utilizing antisera to rat lymphocytes. Each lot of Low-Tox®-R Complement is thoroughly tested for high complement activity and low background cytotoxicity to rat lymphocytes.
Main Application – Cytotoxicity assays using RAT lymphocytes.

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Product Units Format
Low-Tox®-H Rabbit Complement
CL3305
2 x 5 Ml Frozen
Low-Tox®-H Rabbit Complement
CL3310
100 Ml Frozen
Low-Tox®-H Rabbit Complement
CL3331
10 x 1 Ml Lyophilized
Low-Tox®-H Rabbit Complement
CL3335
2 x 5 Ml Lyophilized
Low-Tox®-M Rabbit Complement
CL3051
5 x 1 Ml Lyophilized
Low-Tox®-R Complement
CL3061
3 x 1 Ml Lyophilized
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