QuantideX® NGS Pan Cancer

The QuantideX NGS Pan Cancer Kit* is a next generation sequencing (NGS) based workflow that incorporates both a multiplexed, gene-specific PCR panel as well as supporting workflow elements, including the analytical and reporting features within one comprehensive kit.

The kit interrogates 46 gene regions (amplicons) within 21 genes deemed to be of high clinical significance and potentially actionable* content in various human cancers. The scope of variants reported by the panel includes>1,600 known COSMIC variants, including single nucleotide variants (SNVs), insertions-deletions (indels) and structural rearrangements targeted by the panel.

The kit includes all necessary reagents for pre-analytical DNA QC/quantification, gene-specific PCR, library purification and quantification for sequencing with the Illumina chemistry.

The modular kit design, comprising all necessary library preparation reagents and an easy-to-use push-button analysis and reporting tool, provides a “sample-to-answer” solution tailored to researchers wishing to adopt an NGS-based test with minimal startup costs, regardless of NGS experience.

The Most Clinically-Relevant* Gene Content
21 genes, 46 gene regions of current and emerging targets of clinical significance in human cancer

KIT COMPONENTS

The Kit provides a comprehensive NGS workflow solution including sample QC, targeted enrichment, positive controls, index codes, library purification, library quantification and an easy-to-use bioinformatics reporting tool.

QuantideX qPCR DNA QC Assay
A multiplexed qPCR assay for sample QC and quantification that measures the absolute copy number of PCR-amplifiable DNA in a sample and reports PCR inhibition, guiding smarter sample input. Functional copy number data is later integrated into the variant reporting software providing sample-awareness that dramatically increases call accuracy.

QuantideX Pan Cancer Panel
Gene content selected for clinical relevance* according to NCCN guidelines. Reliably sequence low quantity FFPE/FNA samples with as little as 10ng of DNA input.
Built-in controls including a true FFPE tumour control are included with the kit.

QuantideX Codes
The kit comes equipped with ready-to-use, dual-index barcodes. The index codes are pre-formulated with other primers to support simple master mix preparation and sample labelling integrity. Also included are custom sequencing primers and diluent to support MiSeq™ analysis.

QuantideX Library Pure Prep
The kit integrates library purification reagents that provide size selection using IP magnetic bead chemistry for reliable library quality.

QuantideX Library Quant
Includes library quantification reagents that enable accurate assessment of purified libraries using a calibration-curve free method based on competitive quantitative PCR.

QuantideX NGS Reporter
A sample-to-answer data analytics and reporting suite that delivers efficient, annotated variant analysis without the heavy investment in bioinformatics infrastructure and expertise.

CAPTURING THE COMPLEXITY OF NGS IN ONE SIMPLIFIED WORKFLOW

CONSISTENT COVERAGE ACROSS MULTIPLE SAMPLES AND OPERATORS

QuantideX® NGS Reporter

Push-button analytics & reporting suite – Set up and go workflow designed for easy installation and implementation, right out of the box.

Runs directly on lab desktop – Install locally on a standard Windows® desktop computer. No prior bioinformatics experience or large server environments required.

Comprehensive reporting – Full bioinformatics and reporting of variants (SNPs and indels), and standard QC metrics are automatically calculated.

Integrates QuantideX qPCR DNA QC Assay – “Sample-aware” bioinformatics that factor in functional template copy number, dramatically reducing false call rates.

Seamless updates offered with new panel designs & variant annotation – Software updates allow new panels to be adopted without re-investing in analytics when incorporating

Asuragen QuantideX NGS panels.