QuantideX® NGS RNA Lung Cancer Kit

Product Description

  The QuantideX® NGS RNA Lung Cancer Kit* is a clinical research tool enabling the simultaneous assessment of fusions, exon skipping, and other expression targets frequently observed in non-small cell lung cancer (NSCLC). Leveraging Asuragen’s proprietary NGS-in-a-Box™ workflow and Sample-Aware™ bioinformatics quality control solutions, this kit offers a simple, sensitive, and reliable NGS assay for routine investigation of NSCLC samples.

Features & Benefits

The QuantideX NGS RNA Lung Cancer Kit offers a unique NGS-in-a-Box™ solution that provides unprecedented NGS workflow efficiency and high sensitivity at low input amounts from precious FFPE NSCLC samples.

Reduced Complexity | Assay incorporates sample-to-data solutions in a unique NGS-in-a-Box™ configuration

  • Single assay for a broad range of important NSCLC fusion targets
  • All-inclusive reagents from reverse transcription to NGS-ready library
  • Fully integrated data analysis pipeline

Optimized Workflow | Efficient workflow leads to lower operational costs and reduced hands-on and total turnaround time*

  • Reduced labor vs. current commercially available kits
  • Improved turnaround time enables higher throughput

Quality Performance | Highly sensitive assay with integrated, Sample-Aware™ bioinformatics software and built-in quality checks to minimize erroneous results and sample failures

  • Highly sensitive detection of RNA-based fusions
  • Low input (~20ng) of RNA from FFPE
  • Sample-Aware™ bioinformatics analysis and sample quality control

Relevant Content | A focus on only the most important lung cancer fusion genes, MET exon 14 skipping events, 3’/5′ imbalances and mRNA expression from multiple published data sources such as COSMIC, ClinicalTrials.gov, NCCN Guidelines, etc.

  • 107 NSCLC-relevant fusions
  • ALK, RET, ROS1, NTRK1 & PDGFRA 3’/5’ imbalances
  • MET exon 14 skipping events (e13/e14; e14/e15; e13/e15)
  • 23 mRNA targets, plus 3 endogenous control transcripts

A Fully Integrated Workflow | A unique NGS-in-a-Box™ configuration offers a simplified and fully integrated NGS workflow with cGMP-manufactured reagents, components and controls ready to run right out of the box.


  • RT-PCR reagents
  • A unique RNA Lung Cancer Sample QC kit (QuantideX qPCR RNA Assay (Lung))
  • Gene-specific PCR primers and Master Mix reagents
  • Barcodes for sample multiplexing
  • Library purification and quantification reagents
  • Integrated data analysis and reporting software (QuantideX NGS Reporter)

Rapid & Efficient | Adopt and run NGS-based analysis with minimum investment of time and resources, regardless of NGS experience and infrastructure.

  • Fully integrated workflow reduces complexity and ensures reliable reagent quality
  • Push-button analysis & reporting makes bioinformatics easy and efficient, regardless of experience level
  • Integrated kit reduces workflow time by over 50% than competing NGS panels

Performance Data | QuantideX NGS Assay is capable of detecting fusions and splice variants down to 1:100 cells. (a) Admixture of MET exon 14 positive cell line in the background of wild-type cells, and (b) fusion, and (c) 3’/5’ imbalance status for an admixture of EML4-ALK positive FFPE in the background of a negative FFPE.

Figure 3a

Figure 3b

Figure 3c

Push-button analytics & reporting suite | Set up and go workflow designed for easy installation and implementation, right out of the box.

Runs directly on lab desktop | Install locally on a standard Windows® desktop computer. No prior bioinformatics experience or large server environments required.

Comprehensive reporting | Full bioinformatics and reporting of variants (SNPs and indels), and standard QC metrics are automatically calculated.

Integrates QuantideX qPCR DNA QC Assay | “Sample-aware” bioinformatics that factor in functional template copy number, dramatically reducing false call rates.

Seamless updates offered with new panel designs & variant annotation | Software updates allow new panels to be adopted without re-investing in analytics when incorporating Asuragen QuantideX NGS panels.

*For Research Use Only. Not for use in Diagnostic procedures.

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