We perform HLA Typing using two alternative methods to meet your requirements:

● PCR-RSSO to provide intermediate-resolution HLA genotyping using Luminex xMAP® technology (FLEXMAP 3D);

● Next-generation sequencing (NGS) to provide high-resolution HLA genotyping, using Illumina sequencing technology.

*Please note that our HLA Typing service is for research use only, to support projects and academia for institutions and business; not individual clinical or personal samples.

Bespoke HLA Typing Services Tailored to your Research

When you contact us, we begin a conversation to determine which level of HLA typing is best suited to your project and help you navigate through the complexity of the HLA genetic system.

  • We perform genotyping of the following Classical HLA genes: HLA-A, HLA-B, HLA-C, HLA-DRB1, HLA-DRB3, HLA-DRB4, HLA-DRB5, HLA-DQA1, HLA-DQB1, HLA-DPA1, HLA-DPB1.
  • We accept a variety of different sample types, from cell lines to gDNA.
  • We deliver your results in as few as 48 hours

Data reporting that is tailored to your needs

HLA typing data can be reported in a variety of different formats to suit your needs. Given the complexity of HLA nomenclature we welcome queries to help you better understand what your data means.

Intermediate Resolution HLA Genotyping

Performed by PCR-RSSO, this methodology offers a flexible solution:

  • Any number of samples / gene-sets can be processed, e.g. one sample for HLA-A.
  • PCR-RSSO is particularly cost-effective when typing selected HLA genes, or when ad hoc / low-throughout testing is required.
  • Results are typically reported within 10 working days. Expedited HLA typing can be performed in as little as 48-hours.
  • HLA typing data can be reported in a variety of different formats to suit your needs.

High Resolution HLA typing

High-resolution HLA genotyping aims to unambiguously determine the amino acid sequence across the antigen-binding site of the HLA molecule, where the primary interaction with T lymphocytes occurs.

We perform high-resolution HLA genotyping by next-generation sequencing (NGS):

  • Our multi-gene approach provides HLA genotypes for all Classical HLA genes in one single reaction.
  • A Final Allele Assignment is reported to three-fields.
  • High-resolution genotyping of all Classical HLA genes requires only 50 ng gDNA.
  • Expedited HLA genotyping can be performed in as little as 10 working days.
  • HLA typing data can be reported in a variety of different formats to suit your needs.
hla typing