We perform Human Leukocyte Antigen (HLA) typing using two alternative methods to meet your requirements:
PCR-RSSO to provide intermediate-resolution HLA typing, and next-generation sequencing to provide high-resolution HLA typing.
*For research use only
We work in partnership with you
When you contact us, we begin a conversation to determine which level of HLA typing is best suited to your project and help you navigate through the complexity of the HLA genetic system.
- We perform genotyping of the following Classical HLA genes: HLA-A, HLA-B, HLA-C, HLA-DRB1, HLA-DRB3, HLA-DRB4, HLA-DRB5, HLA-DQA1, HLA-DQB1, HLA-DPA1, HLA-DPB1.
- We accept a variety of different sample types, from cell lines to gDNA.
- We deliver your results in as few as 48 hours
Intermediate Resolution HLA typing
Performed by PCR-RSSO, this methodology offers a flexible solution:
- Any number of samples / gene-sets can be processed, e.g. one sample for HLA-A.
- PCR-RSSO is particularly cost-effective when typing selected HLA genes or ad hoc / low-throughout testing is required.
- Results are delivered within 10 working days, but expedited HLA typing can be performed in as little as 48 hours.
- HLA typing data can be reported in a variety of different formats to suit your needs.
High Resolution HLA typing
Aims to unambiguously determine the amino acid sequence across the antigen-binding site of the HLA molecule where interaction with T lymphocytes occurs.
We perform high-resolution HLA typing by next-generation sequencing:
- Our multi-gene approach provides HLA genotypes for all classical HLA genes in one single reaction.
- High-resolution genotyping of all HLA genes requires only 50 ng gDNA.
- Expedited HLA typing can be performed in as little as 10 working days.
- HLA typing data can be reported in a variety of different formats to suit your needs.
