Browser Warning
VHBio is not supported on Internet Explorer and will not provide you with the best experience. Please consider switching to a compatible browser such as Microsoft Edge, Google Chrome or Firefox.
We genuinely believe in what we do and want to be the best life sciences supply partner. Any information to help our journey is very welcome. Please take a few minutes to help us improve and make your future experiences better:
The mHypoA-Kiss/GFP cell lines were generated using a proprietary platform technology enabling the creation of pools of immortalized hypothalamic neurons. Kiss/GFP transgenic mouse (Kiss-1tm1.1(cre/EGFP)Stei/J; strain (129S6/SvEvTac x C57BL/6NCr)F1) hypothalami were micro-dissected from 2-month old female or male mice isolating the ARC and AVPV nuclei individually for the female mice or using the entire hypothalamus for […]
The mHypoA-Kiss/GFP cell lines were generated using a proprietary platform technology enabling the creation of pools of immortalized hypothalamic neurons. Kiss/GFP transgenic mouse (Kiss-1tm1.1(cre/EGFP)Stei/J; strain (129S6/SvEvTac x C57BL/6NCr)F1) hypothalami were micro-dissected from 2-month old female or male mice isolating the ARC and AVPV nuclei individually for the female mice or using the entire hypothalamus for the male mice. These mice contain an eGFP transgene driven by the Kiss-1 promoter. These hypothalamic primary cultures were immortalized by retroviral transfer of the SV40 T-Ag. The four immortalized hypothalamic pools were sorted on a cell sorting flow cytometer based on GFP fluorescence. These cells represent the entire population of mHypoA-Kiss/GFP neurons from the ARC (mHypoA-Kiss/GFP-3) and AVPV (mHypoA-Kiss/GFP-4) for the female and all Kiss neurons for the male (mHypoA-Kiss/GFP-1-2) and have not been further sub-cloned.
These cells have been found to express an ever expanding array of neuropeptides, enzymatic markers and biologically active receptors. As such, these cells enable accurate in-vitro assays for use in the discovery, development and validation of new therapeutics targeted to central-nervous system diseases and disorders, including obesity, stress, and metabolic disorders, amongst others.
Product | Units |
---|---|
mHypoA-Kiss/GFP-1 (male) CLU505 |
1 Ml |
mHypoA-Kiss/GFP-2 (male) CLU506 |
1 Ml |
mHypoA-Kiss/GFP-3 (female; ARC nuclei derived) CLU507 |
1 Ml |
mHypoA-Kiss/GFP-4 (female; AVPV nuclei derived) CLU508 |
1 Ml |
We work with a range of suppliers with novel and innovative products sourced from our global partners.
Find Products