Adult Mouse Kiss/GFP Cell Lines

The mHypoA-Kiss/GFP cell lines were generated using a proprietary platform technology enabling the creation of pools of immortalized hypothalamic neurons. Kiss/GFP transgenic mouse (Kiss-1tm1.1(cre/EGFP)Stei/J; strain (129S6/SvEvTac x C57BL/6NCr)F1) hypothalami were micro-dissected from 2-month old female or male mice isolating the ARC and AVPV nuclei individually for the female mice or using the entire hypothalamus for the male mice. These mice contain an eGFP transgene driven by the Kiss-1 promoter. These hypothalamic primary cultures were immortalized by retroviral transfer of the SV40 T-Ag. The four immortalized hypothalamic pools were sorted on a cell sorting flow cytometer based on GFP fluorescence. These cells represent the entire population of mHypoA-Kiss/GFP neurons from the ARC (mHypoA-Kiss/GFP-3) and AVPV (mHypoA-Kiss/GFP-4) for the female and all Kiss neurons for the male (mHypoA-Kiss/GFP-1-2) and have not been further sub-cloned.
These cells have been found to express an ever expanding array of neuropeptides, enzymatic markers and biologically active receptors. As such, these cells enable accurate in-vitro assays for use in the discovery, development and validation of new therapeutics targeted to central-nervous system diseases and disorders, including obesity, stress, and metabolic disorders, amongst others.