One Lambda™ MagSort™

Targeted isolation of HLA antibodies from human sera using adsorption/elution via HLA-specific antigen-coated magnetic beads

Key Features and Benefits

HLA-antibody isolation

• Isolation of individual HLA antibodies of interest (e.g. donor specific antibody) to explore antibody properties, including through downstream cellular (e.g. flow cytometric crossmatch) and solid-phase assays (e.g. LABScreen Single Antigen & Single Antigen ExPlex).
• Enhancing eplet-level HLA-antibody specificity analysis, supported by HLA Matchmaker functionality within HLA Fusion software.  Includes potential for novel eplet discovery based on unique antibody binding patterns.
• Assessment of HLA eplet immunogenicity.
• Distinguishing genuine reactivity from background reactivity and aiding in identification of weak reactivity.
• Determining polyclonal or monoclonal nature of HLA-antibody profile.
• In specific cases, indirect assessment of relative HLA-antibody quantity and binding strength.

Customisable panels

Efficient workflow

• Utilises standard laboratory equipment.
• Provides immediate antigen availability as compared to alternative cell-based antibody adsorption/elution methodologies.
• Has a hands-on time of up to 45 minutes and time to completion of 3.5 hours.

The use of HLA eplets to interpret HLA-antibody data is becoming more commonplace.  However, in complex antibody profiles, confident identification of one or more specific eplet targets of reactivity can be challenging.  One Lambda™ MagSort™ enables isolation of targeted HLA-antibodies from complex serum providing opportunity for improved characterisation, adding precision to HLA-antibody data interpretation and its application in a transplant setting.

Get in touch below with any questions about One Lambda™ MagSort™ or to place an order. 

Find out more

• What’s New In Antibody…?
• Vu et al. Using MagSort to identify eplets in a high PRA patient serum and revealing a novel HLA-A*01:01 specific binding pattern. Hum Immunol. 2025 Jul 19;86(5):111344

• Vu et al. Using single antigen specificity magnetic beads for the isolation of specific antibodies against HLA antigens. HLA. 2024; 103(4):e15490.

• Liwski et al. Cutting through the weeds: Evaluation of a novel adsorption with crossmatch cells and elution protocol to sharpen HLA antibody identification by the single antigen bead assay. Front Genet. 2022 Nov 30;13:1059650.

• Webinar: Study of HLA Eplet immunogenicity with MagSort Beads. Magali Devriese, Hôpital St Louis, Paris