Find Products

AmplideX® DM1 Dx Kit

The AmplideX® DM1 Dx Kit introduces a groundbreaking alternative for the detection and sizing of CTG trinucleotide repeats within the DMPK gene. Leveraging the Repeat-Primed PCR (RP-PCR) design in a simple, streamlined, PCR-only workflow, the kit provides an opportunity to replace Southern blot technology and put DMPK analysis within the reach of laboratories everywhere. Features […]

The AmplideX® DM1 Dx Kit introduces a groundbreaking alternative for the detection and sizing of CTG trinucleotide repeats within the DMPK gene. Leveraging the Repeat-Primed PCR (RP-PCR) design in a simple, streamlined, PCR-only workflow, the kit provides an opportunity to replace Southern blot technology and put DMPK analysis within the reach of laboratories everywhere.

Features & Benefits

AmplideX technology has revolutionized how repeat expansions within the FMR1, C9orf72, and TOMM40 genes can be analyzed, significantly expediting time-to-result and reducing the reliance on cumbersome Southern blot analysis.  The AmplideX® DM1 Dx Kit extends this groundbreaking approach to the DMPK gene, which has long challenged laboratory analysis due to the presence of very large expansions (over 1000 repeats) and a high degree of mosaicism.  The AmplideX® DM1 Dx Kit, which is for use on widely established CE systems, overcomes these challenges and enables the sensitive detection and sizing of these DMPK repeats all within a single laboratory shift – without the need for Southern blot confirmation.

Reduced Complexity

Analysis of the DMPK gene has been simplified through:

  • Proprietary PCR solutions for GC-rich amplification and detection
  • Eliminates need for Southern blot
  • Resolves zygosity and detects mosaicism

Optimized Workflow

Valuable operator hands-on time has been significantly reduced through:

  • Direct injection of PCR products into CE instruments
  • Fully kitted, end-to-end solutions that significantly reduce hands-on-time
  • Sample-to-result possible within a single shift

Quality Performance

Performing DMPK analysis with greater sensitivity and accuracy:

  • Accurate sizing of all alleles ≤200 CTG repeats and detection of all alleles >200 repeats using CE
  • Optional AGE protocol allows sizing of alleles between 200-2000 repeats
  • Converts raw base pair data into number of repeats

Analytical Characteristics

Analytical characteristics of AmplideX® DM1 Dx Kit:

  • High degree of concordance to Southern blot for large expansions (Figure 1)
  • Accurate sizing of expansions up to 200 repeats (Figure 2)
  • Clearly resolve zygosity (Figure 3)

Table 1. Comparison of residual clinical sample results between Asuragen (AmplideX® DM1 Dx Kit) and partner lab (PCR Southern blot).

Figure 1. Results of expanded allele analysis using AmplideX AGE protocol.

Figure 2: Sensitive and accurate sizing of DMPK expansions up to 200 repeats

Figure 3: Clear resolution of zygosity.

Figure 4: Workflow for the AmplideX® DM1 Dx Kit

Read more +

View Products Enquire Now

Single product

Filter by:

Product Units
AmplideX® DM1 Dx Kit
676048
32 Reactions
  • Enquire Now

    We work with a range of suppliers with novel and innovative products sourced from our global partners.

  • This field is for validation purposes and should be left unchanged.

Find Products

We work with a range of suppliers with novel and innovative products sourced from our global partners.

Find Products

PRODUCT AVAILABILITY UPDATE

At VH Bio we remain wholeheartedly dedicated to continuing to serve as your reliable and trusted supply partner, to allow you to focus on the essential work you do.

Both VH Bio and our supply partners continue to be fully operational during the COVID-19 outbreak. We are in regular contact with our suppliers worldwide to ensure continued product availability and as this global situation evolves, we will take all steps necessary to continue providing our customers with high-quality products, information and support.