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The AmplideX® DM1 Dx Kit introduces a groundbreaking alternative for the detection and sizing of CTG trinucleotide repeats within the DMPK gene. Leveraging the Repeat-Primed PCR (RP-PCR) design in a simple, streamlined, PCR-only workflow, the kit provides an opportunity to replace Southern blot technology and put DMPK analysis within the reach of laboratories everywhere. Features […]
The AmplideX® DM1 Dx Kit introduces a groundbreaking alternative for the detection and sizing of CTG trinucleotide repeats within the DMPK gene. Leveraging the Repeat-Primed PCR (RP-PCR) design in a simple, streamlined, PCR-only workflow, the kit provides an opportunity to replace Southern blot technology and put DMPK analysis within the reach of laboratories everywhere.
AmplideX technology has revolutionized how repeat expansions within the FMR1, C9orf72, and TOMM40 genes can be analyzed, significantly expediting time-to-result and reducing the reliance on cumbersome Southern blot analysis. The AmplideX® DM1 Dx Kit extends this groundbreaking approach to the DMPK gene, which has long challenged laboratory analysis due to the presence of very large expansions (over 1000 repeats) and a high degree of mosaicism. The AmplideX® DM1 Dx Kit, which is for use on widely established CE systems, overcomes these challenges and enables the sensitive detection and sizing of these DMPK repeats all within a single laboratory shift – without the need for Southern blot confirmation.
Analysis of the DMPK gene has been simplified through:
Valuable operator hands-on time has been significantly reduced through:
Performing DMPK analysis with greater sensitivity and accuracy:
Analytical characteristics of AmplideX® DM1 Dx Kit:
Table 1. Comparison of residual clinical sample results between Asuragen (AmplideX® DM1 Dx Kit) and partner lab (PCR Southern blot).
Figure 1. Results of expanded allele analysis using AmplideX AGE protocol.
Figure 2: Sensitive and accurate sizing of DMPK expansions up to 200 repeats
Figure 3: Clear resolution of zygosity.
Figure 4: Workflow for the AmplideX® DM1 Dx Kit
Product | Units |
---|---|
AmplideX® DM1 Dx Kit 676048 |
32 Reactions |
20th September 2018
Categories: DMPK Analysis, Myotonic Dystrophy Type I (DM1)
25th September 2018
Categories: DMPK Analysis, Myotonic Dystrophy Type I (DM1)
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