Mouse Suprachiasmatic Nucleus

Cedarlane Mouse Suprachiasmatic Nucleus Cell Line (mHypoA-SCN mix) 

Immortalised Hypothalamic Neurons for Circadian Rhythm and Neuroendocrine Research 

Cedarlane’s mHypoA-SCN mix is an immortalised cell line derived from the suprachiasmatic nucleus (SCN)—the central circadian clock of the mammalian brain. Developed using proprietary platform technology, this line provides a reliable in vitro model for studying circadian regulation, neuropeptide expression, and neuroendocrine signalling. 

Product Overview 

• Source: Primary SCN neurons microdissected from six adult female C57BL/6 mice 

• Immortalisation Method: Retroviral transfer of SV40 T-antigen into pooled primary cultures 

• Sub-cloning: None performed – cell line maintains natural heterogeneity 

• Designation: mHypoA-SCN mix 

Key Features and Benefits 

• Derived specifically from the suprachiasmatic nucleus – the brain’s master circadian clock 

• Expresses a growing array of neuropeptides, enzymatic markers, and functional receptors 

• Enables high-throughput, reproducible in vitro assays for CNS drug discovery and mechanistic studies 

• Suitable for exploring neuroendocrine pathways, metabolic regulation, stress responses, and circadian rhythm disorders 

Applications 

• Circadian biology and molecular clock studies 

• Neuropeptide signalling and hypothalamic function 

• Stress and metabolic disorder research 

• CNS drug screening and target validation 

This cell line is a powerful model for researchers studying SCN-regulated physiological processes and central nervous system diseases involving disrupted hypothalamic function. 

Technical Documentation 

Download the full Adult Mouse Hypothalamic Cell Line mHypoA-SCN mix Data Sheet and Adult Mouse Hypothalamus Cell Line (mHypoA-SCN mix) Screen Profile mHypoA-SCN Cell Line Screening for additional molecular and functional characterisation.