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AmplideX® PCR/CE HTT Kit

The AmplideX PCR/CE HTT Kit* makes the detection and sizing of CAG trinucleotide repeats in the HTT gene easier than ever. In just a single PCR reaction, the assay overcomes the challenges of adjacent SNPs and variable CCG repeats to accurately and reliably report the number of repeats in one day, from as little as […]

The AmplideX PCR/CE HTT Kit* makes the detection and sizing of CAG trinucleotide repeats in the HTT gene easier than ever. In just a single PCR reaction, the assay overcomes the challenges of adjacent SNPs and variable CCG repeats to accurately and reliably report the number of repeats in one day, from as little as 10ng gDNA.

Features & Benefits

The AmplideX PCR/CE HTT Kit includes reagents to perform a two-primer, anchor-primed PCR of the HTT CAG repeat region followed by fragment sizing by capillary electrophoresis. This single-tube PCR reaction provides a streamlined workflow, enabling sample-to-result in approximately 6 hours using readily available laboratory equipment.

Reduced Complexity

  • Proprietary PCR solutions for GC-rich amplification and detection
  • Eliminates need for multiple PCRs – one result, straightforward analysis
  • Resolves zygosity and detects large expansions

Optimized Workflow

  • Fully kitted, end-to-end solutions that significantly reduce hands-on-time
  • Sample-to-result possible within a single shift
  • Identical PCR and CE conditions as the AmplideX PCR/CE DMPK Kit

Quality Results

  • Reliable, unambiguous results and a robust stutter peak pattern
  • Accurate sizing across the entire CAG repeat range
  • Software converts raw base pair data into number of repeats

*For Research Use Only. Not for use in diagnostic procedures.

Analytical Characteristics

Excellent Concordance with Previously Characterized Samples (Table 1)
Repeat profile is conserved across known SNPs (Figure 1)
Clearly resolves zygosity (Figure 2)

Table 1. Comparison of CAG repeat alleles in commercially available samples, as reported by Kalman et al. (2007)

Figure 1. Repeat profile is conserved across known SNPs.

Figure 2. Zygosity is clearly resolved using the AmplideX PCR/CE HTT Kit.

Figure 3. Workflow for the AmplideX PCR/CE HTT Kit.

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AmplideX® PCR/CE HTT Kit
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