LinkSēq HPA and ABO Typing Kits

LinkSēq Portfolio for Blood Genotyping 

The LinkSēq real-time PCR molecular antigen typing kits – LinkSēq HPA and ABO Typing Kits – overcome the major challenges of molecular typing by increasing laboratory productivity, reducing turnaround time, and predicting phenotypes for many antigens given the common variants for those antigens. 

Key Benefits of LinkSēq 

• Fast workflow with minimal hands-on effort: 10 minutes hands-on time – simply add your DNA to the pre-aliquoted buffer/enzyme mix, add to the tray, seal, and you are done. DNA sample to results in 90 minutes, 

• Accurate real-time PCR: Allele-specific amplification combined with SYBR Green to detect PCR products without chips or gels. 

• Robust contamination protection: Samples are handled only once and then sealed in reaction trays. Increased sample protection as amplified PCR products remain sealed. 

• Easy data analysis via SureTyper Blood: Automatically calls genotype and predicts phenotypes based on ISBT allele database and proprietary algorithm – in seconds. Simple data drill-down tools and easy-to-use, intuitive interface.  

LinkSēq™ ABO Typing Kits 

ABO alleles encode the enzyme Glycosyltransferase, which adds sugar groups to the surface of red blood cells (RBC) and other cells. ABO is implicated in tissue rejection as well as adverse transfusion reactions. 

Many A subtypes involve a weakly expressed A antigen. Subtypes have a slight structural variation that can be recognised through routine serological testing. 

• 196328R types eight samples per 384-well tray. All necessary reagents are included in the kit. 

• 196321R types one sample per 384-well tray. All necessary reagents are included in the kit. 

• 196022R types two samples per 96-well tray. All necessary reagents are included in the kit. 

• 196021R types one sample per 96-well tray. All necessary reagents are included in the kit. 

LinkSēq™ HPA Typing Kits 

LinkSēq Human Platelet Antigen (HPA) Typing trays come in both 384- and 96-well format. 

Human Platelet Antigens (HPA) are glycoproteins expressed on platelet membranes. HPA can stimulate the production of alloantibodies after transfusion. In subsequent blood transfusions, alloantibodies against donor HPA may cause immunological reactions in the recipient’s blood, resulting in the lysis of the transfused platelets and thrombocytopenia. Because of the possibility of such immunological reactions, HPA typing is important in the transfusion setting. 

The LinkSēq HPA kits provide HPA typing for HPA-1 through HPA-11, and HPA 15. 196308C types 8 samples per 384-well tray. All necessary reagents are included in the kit. 

196302C types 2 samples per 384-well tray. All necessary reagents are included in the kit. 

 196002C types 2 samples per 96-well tray. All necessary reagents are included in the kit. 

 196001C types 1 sample per 96-well tray. All necessary reagents are included in the kit. 

LinkSēq ABO Typing Trays and LinkSēq Human Platelet Antigen (HPA) Typing Trays come in both 384- and 96-well format.