SepsiTest-UMD

Benefits:

• One protocol for a broad range of clinical specimens (fluid, tissue, biofilm)
• Detection of viable, non-culturable and antibiotic-inhibited pathogens
• Extraction of microbial DNA included
• Precise identification of bacterial and fungal species by Sanger sequencing
• PCR and extraction controls included
• All reagents guaranteed DNA-free

Application:

Culture-independent molecular diagnosis of bacterial and fungal pathogens in specimens.

Extraction:

Enrichment of pathogens – pathogen DNA isolation

Assays:

16S and 18S rRNA gene PCR or Real-Time PCR – primers included for Sanger sequencing analysis – PCR and extraction controls

Specimens:

EDTA blood – blood culture – CSF – BAL – sputum – joint aspirates – ascites fluid – peritoneal fluid – pus – wound and nasal swabs – bone and soft tissue biopsies – heart and aortic valves and more.