One Lambda Devyser Chimerism Assay

NGS-based chimerism analysis with unmatched sensitivity and simplicity

The One Lambda Devyser Chimerism Assay is an advanced, CE-IVD marked next-generation sequencing (NGS) solution for monitoring donor-recipient chimerism following allogeneic stem cell transplantation.

Designed for use with Illumina platforms, the assay enables ultrasensitive and accurate detection of microchimerism, an early sign of disease relapse, with detection limits down to 0.05%.

This all-in-one solution offers an integrated workflow; PCR amplification, sample indexing, and sequencing-ready library prep, all achieved with less than 45 minutes hands-on time. The streamlined Advyser Chimerism software automatically determines chimerism ratios from sequencing data (roughly 1 minute per sample), ensuring robust, reproducible results with minimal user interaction required. This 1-day workflow means that standard and urgent reporting timelines are easily met, supporting timely clinical decision-making.

Key Features and Benefits:

• Ultrasensitive detection of minor DNA components down to 0.05%
• Superior specificity using highly informative indel markers for accurate discrimination
• Simplified workflow with few manual steps and ready-to-use reagents
• Rapid turnaround meeting standard (≤5 working days) and urgent (≤3 working days) reporting targets
• Multiplexed NGS-based assay so multiple patients can be processed per run
• Automated, user-friendly software for intuitive interpretation and reporting

Workflow summary:

• Sample Collection & DNA Extraction: genomic DNA from patient and donor or post-transplant sample from recipient
• Multiplex PCR Amplification: targeting informative indel markers
• Library Preparation: Rapid, sequencing-ready library using included reagents
• NGS Sequencing: compatible with Illumina instruments
• Data Analysis: Automated, intuitive software for chimerism calculation and reporting

The future of chimerism monitoring is here! With unparalleled sensitivity, specificity, and ease of use, the One Lambda Devyser Chimerism Assay allows transplant teams to detect relapse and rejection earlier for more proactive, personalised patient care.

Read more:

The Next Generation of Post-Transplant Chimerism Monitoring

Minority Report: Why detecting microchimerism can make the biggest difference