Lympholyte®-R (Rat)

Isolation of Viable Rat Lymphocytes from Lymphoid Tissue

CEDARLANE’s Lympholyte®-Rat Cell Separation density gradient centrifugation media can be utilized with a simple protocol for the elimination of erythrocytes, dead cells and debris from rat spleen, lymph node, thymus and bone marrow suspensions. The resulting cell population demonstrates a high and non-selective recovery of viable lymphocytes, which are suitable for use as target cells in cytotoxicity and FACS assays, as well as in in vivo and in vitro functional studies. Other successful applications include:

• The removal of dead cells in sequential cytotoxicity studies eg. B-cell depletion.
• The removal of erythrocytes, dead cells and debris from other rat tissue suspensions including liver and lung.
• The harvesting of viable cells and removal of dead cells and debris from various clone cell and hybridoma cell lines.