FocusClear™ Tissue Clearing Agent for High-Resolution Imaging

FocusClear™ – Tissue Clearing Solution for Deep Optical Imaging 

Ready-to-Use Clearing Agent for Fluorescence Microscopy, Immunohistochemistry, and Whole-Mount Imaging 

FocusClear™ is a powerful, non-toxic, water-soluble tissue clearing agent designed to significantly enhance the transparency of fixed biological specimens—dramatically improving optical imaging depth and clarity. Suitable for use in a wide range of fluorescent and non-fluorescent labelling techniques, FocusClear™ enables high-resolution visualisation of internal structures up to 500 μm below the tissue surface. 

🔬 Key Features and Benefits 

• Deep tissue visualisation: Penetrates up to 500 μm beneath the specimen surface 

• Doubles imaging depth compared to traditional glycerol-based mounting media 

• Enhances laser excitation and improves optical signal detection for both colourimetric and fluorescent samples 

• Compatible with immunofluorescence, in situ hybridisation, whole-mount IHC, and fluorescent protein-labelled tissues 

• Works with lipophilic dyes (e.g., DiI, DiD, NBD-ceramide) 

• No aliquoting, mixing, freezing, or centrifugation required – always liquid and ready to use 

• Room temperature storage for convenience and long-term usability 

🧪 Applications 

• Fluorescence microscopy 

• Confocal and laser scanning microscopy 

• Whole-mount and thick tissue imaging 

• Neuroanatomy, developmental biology, and immunohistochemistry 

• Ideal for single cells, tissues, and complex structures like brain slices or organoids 

In comparative studies, such as on American cockroach brain tissue (1,000 μm thick), FocusClear™ outperformed both PBS and glycerol, producing clearer, more transparent samples within just one hour of treatment. 

📦 Why Choose FocusClear™? 

FocusClear™ simplifies your workflow while enhancing image quality, signal detection, and optical clarity. Samples can be easily transferred from common storage media (e.g., water, buffers, alcohol, DMSO, DMF, or glycerol) directly into FocusClear™ for immediate use—making it an essential tool for labs seeking high-performance clearing without complex preparation steps.