Taq DNA Polymerase

Taq DNA Polymerase is a highly purified recombinant thermostable DNA polymerase that has been isolated from E. coli carrying a vector encoding the Thermus aquaticus DNA polymerase gene. The enzyme possesses a highly processive 5’-3’ DNA polymerase activity with optimum activity achieved at 74°C.

It exhibits high thermal stability in withstanding prolonged incubations at elevated temperatures (95°C). Taq DNA Polymerase lacks 3’-5’ exonuclease activity.

Red Taq DNA Polymerase contains an inert red dye which allows identification of the reactions which contain enzyme. The dye has no adverse effect on PCR.

Green reaction buffer contains two dyes (blue and yellow) that separate during electrophoresis to monitor migration progress. Reactions assembled with Green reaction buffer have sufficient density for direct loading onto agarose gels. Green reaction buffer is recommended for any amplification reaction that will be visualized by agarose gel electrophoresis and ethidium bromide staining. The dyes absorb between 225–300nm, making standard A260 readings to determine DNA concentration unreliable.