Hot Start DNA Polymerase

Hot Start DNA Polymerase is recommended for high specificity PCR reactions. The thermal activation prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during PCR set up.

Hot Start DNA Polymerase is a highly purified recombinant thermostable DNA polymerase that has been isolated from E. coli carrying a vector encoding the Thermus aquaticus DNA polymerase gene. The enzyme possesses a highly processive 5’-3’ DNA polymerase activity with optimum activity achieved at 74°C. It exhibits high thermal stability in withstanding prolonged incubations at elevated temperatures (95°C). Hot Start DNA Polymerase lacks 3’-5’ exonuclease activity.

Red Hot Start DNA Polymerase contains an inert red dye which allows identification of the reactions which contain enzyme. The dye has no adverse effect on PCR.