Plant: Stone Pine Needle Prepared Microscope Slide

Versatile Autofluorescent Slide for Plant Imaging, Spectral Detection, and Deep Tissue Demonstration 

The OptiSlides Stone Pine Needle Slide is a high-performance, autofluorescent plant slide designed to demonstrate and validate advanced microscope capabilities across confocal, multiphoton, and widefield imaging systems. Developed by scientists for scientists, this slide replaces the classic Convallaria slide with a more versatile and spectrally rich alternative. 

Exceptional Autofluorescence Across the Spectrum 

The pine needle’s natural structure is packed with endogenous fluorophores, producing broad-spectrum autofluorescence from UV to Near Infrared (NIR). This makes it an ideal tool to: 

• Showcase deep tissue penetration with confocal and multiphoton microscopy 

• Demonstrate spectral detection and unmixing capabilities 

• Test multi-colour image registration and stitching on widefield slide scanners and confocal systems 

Universally Compatible and Ready to Use 

Compatible with nearly any light microscope, including brightfield, widefield, darkfield, polarised light, confocal, and multiphoton platforms, this slide is ready for use straight out of the box. Its uniform tissue structure and large format make it easy to locate and reimage distinct features in each channel. 

Technical Specifications 

• Sample Thickness: Available in up to 400 μm 

• Mounting Medium: VectaShield Plus (liquid), with double-sided adhesive spacer 

• Coverslip: #1.5, sealed with polish or sealant 

• Reusable: Clean gently with 70% ethanol and lens paper for repeated use 

Whether you’re troubleshooting, demonstrating spectral capabilities, or calibrating high-end imaging systems, the Stone Pine Needle Slide delivers consistent, visually striking results across a broad spectral range.