Cell: Mouse Fibroblast Prepared Microscope Slide

Cell Slides for Fluorescent Microscope Calibration, Super-Resolution Imaging, and Training 

OptiSlides Cell Slides are precision-engineered tools designed for scientists and imaging specialists to troubleshoot, calibrate, and demonstrate high-end fluorescence microscope performance. With vibrant, multi-channel fluorescence and sub-resolution targets, these slides are ideal for use across confocal, widefield, multiphoton, and STED super-resolution systems. 

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Advanced Fluorescent Reference Slides for Modern Microscopy 

Each slide features cultured mouse fibroblast cells stained with: 

• DAPI (nuclei) 

• Phalloidin (stress fibres) 

• Peroxisomes (~150 nm, ideal for super-resolution) 

• Plus either Vimentin or Paxillin 

Slides are available in: 

• 4-colour, 4-channel configuration 

• 6-channel format, adding Near Infrared fluorophores (735 nm & 785 nm laser lines) 

These updated 4- and 6-channel formats replace outdated 3-colour reference slides, delivering improved performance for today’s multi-channel fluorescence imaging and spectral detection workflows. 

6-Channel Near Infrared (NIR) Configuration Available 

In addition to the 4-colour format, Mouse Fibroblast slides are available in a 6-channel configuration incorporating Near Infrared (NIR) fluorophores (730 nm and 785 nm excitation). 

This extended format enables: 

• Validation of filter sets and detector performance across visible and NIR wavelengths 

• Chromatic integrity assessment 

• Image registration accuracy in X, Y and Z 

• Sub-resolution target verification 

• Multi-channel alignment and bleed-through evaluation 

Designed for use on confocal, widefield, and NIR-enabled fluorescence microscopy systems. 

Key Applications 

• Microscope setup and troubleshooting 

• Filter and camera alignment validation 

• Chromatic shift checks in X, Y, and Z 

• Super-resolution imaging verification (e.g. STED) 

• Image registration and channel stitching tests 

• Fluorescence microscopy training and education 

Fluorophores & Channels – 6-Channel NIR Variant 

The 6-channel Mouse Fibroblast slide includes: 

This configuration provides robust signal separation across visible and near-infrared channels, enabling validation of high-content and multiplex imaging workflows. 

Built-In Technical Excellence 

• Peroxisomes (~150 nm) serve as sub-resolution targets for testing optical performance at the nanoscale 

• Grown directly on a #1.5 coverslip for optimal imaging compatibility 

• Mounted in Vectashield Vibrance, a hardening medium with a refractive index of 1.47 

• Short-term storage at room temperature in the included OptiSlides case 

• Long-term storage at 4–8°C recommended (not sealed) 

• Reusable: simply clean with ethanol and lens paper for multiple imaging sessions 

Customisation Available 

We are actively developing co-localisation double-stained versions and slides with custom fluorophores optimised for STED super-resolution of peroxisomes.