DNA-Free PCR Master Mixes for Pathogen Detection

PCR and Real-Time PCR Reagents

Molzym’s Mastermix 16S/18S Basic and Mastermix 16S/18S Dye products are designed to deliver exceptional sensitivity and reliability for detecting microbial DNA in PCR assays using your primers. Our advanced purification technology ensures that reagents are DNA-free, guaranteeing precise and accurate results.

Key Features and Benefits:

• High-activity, DNA-free Taq DNA polymerases
• Hot start Taq available for enhanced specificity
• Reliable PCR amplification up to 40 cycles without background interference
• Master mixes optimized for both PCR and Real-Time PCR (fluorescent dye)
• Ready for probe-based assays

Broad-Range 16S & 18S Assays

Molzym’s Mastermix 16S Complete and Mastermix 18S Complete are powerful tools for detecting bacterial and fungal DNA with exceptional precision. These assays amplify hypervariable regions of the 16S and 18S rRNA genes, achieving detection levels down to femtogram quantities.

Key Features and Benefits:

• High-activity, DNA-free reagents
• Targeted amplification of:
  • Bacterial 16S (V3/V4 hypervariable region)
  • Fungal 18S (V8/V9 hypervariable region)
• Reliable amplification up to 40 cycles without background
• Compatible with both PCR and Real-Time PCR (fluorescent dye) assays
• Sequencing primers available for Sanger sequencing

Applications

These highly sensitive and rapid detection tools are ideal for:

• Microbial DNA analysis in clinical diagnostics
• Quality testing of food and pharmaceutical products
• Contamination control in biotechnological processes

Molzym’s PCR and Real-Time PCR products are manufactured under strict quality control to ensure DNA contamination-free reagents, delivering reliable and reproducible results for every test.