Dye Terminator v3.1 Cycle Sequencing Kits

The QuantumDye™ v3.1 Terminator Cycle Sequencing Kit is based on the Sanger Dideoxynucleotide Chain Termination method and has been developed as a direct, drop-in substitute for BigDye® Terminator v3.1.

QuantumDye™ 3.1 Cycle Sequencing Kits

• A direct, drop-in substitute for BigDye® Terminator v3.1, with no changes in protocol, dilution, workflow, calibration or settings using DyeSet Z.
• Same high performance, but at a greater value:
• Identical performance and direct, drop-in substitute to BigDye Terminator v3.1
• Flexible packaging formats
• Fully compatible with all ABI Genetic Analysers
• Uniform peak heights and long, high-resolution cycle sequencing reads with extremely low background
• Optimised for de novo sequencing and resequencing applications
• Formulated as a 2.5x concentrated cycle sequencing mix

The kit is provided with a 2.5x concentrated ready-reaction premix, plus cycle sequencing dilution buffer and controls (plasmid DNA template and sequencing primer) fully optimised for a highly flexible chemistry, designed for all kinds of cycle sequencing applications, including de novo sequencing and resequencing. The kit generates data with uniform peak heights and optimised signal balance to produce long, high-quality reads.

V.H. Bio’s Customer Feedback on QuantumDye™

Our customer, Micropathology Ltd., trialled QuantumDye™, and found the product to have better performance than ABI BigDye and a more streamlined change as compared to Promega, which required changes to the protocol.

Figure 1. Data illustrating live performance of V.H. Bio’s QuantumSeq Dye Terminator Cycle Sequencing Kit in comparison to ABI BigDye and Promega ProDye.

“The three charts show the contiguous read length for four amplicons (HCVG1A, HBV, Mgen23 and CE1) for each of the reagents tested. The Mgen23 amplicon is very short and we have issues producing good quality sequence data with recent batches of BigDye. As you can see from the chart (Mgen23) we obtain superior sequence data when using the QuantumSeq.

“We are now using up our stocks of BigDye and are looking forward to moving over to the QuantumSeq dye for all sequencing based assays.”

– Dr Jennifer Holden, Post-doctoral Scientist, Micropathology Ltd

Sanger Sequencing in Diagnostic Labs

The sequencing of single or multiple genes is often the first step in the genetic diagnosis of a disease and informs subsequent treatment and care. The development of the Sanger sequencing method in 1977 allowed labs to sequence DNA fragments with automated base calls for the first time. While continual developments have led to innovative technologies such as Next Generation Sequencing (NGS), Sanger sequencing is still considered the gold standard technique for some clinical scenarios, for example, 16S sequencing, human leukocyte antigen (HLA) typing, microsatellite instability (MSI), tandem repeats, and rare variant confirmation. 1,2

Download our infographic to:

• Explore the benefits of using Sanger sequencing in diagnostic laboratories
• Compare Sanger vs. NGS Sequencing for Clinical Decisions
• Learn about the key features of QuantumSeq™ Sanger Sequencing Reagents and understand the competitor analysis

The Dye Terminator Cycle Sequencing Kit Components include:

• 5x QuantumDye Cycle Sequencing Mix
• pGEM control DNA
• M13(-21) control primer
• QuantumDye 5x sequencing buffer

Contact VH Bio to discuss how the Dye Terminator Cycle Sequencing Kit can benefit your scientific research and to request your free sample.