Hot-Start and Standard PCR Master Mixes

Rovalab’s 2x PCR Master Mix contains all reagents required for PCR and is designed to make PCR as easy and simple as possible. All components (inclusive Taq DNA Polymerase respectively Hot Start DNA Polymerase) are provided in an optimized concentration in the 2x PCR-Master solution. With 2x PCR Master Mix all you need to do is to add primers and template DNA, thus minimizing the pipetting effort and possible sources of error. The mix is suitable for PCR amplification of DNA-fragments up to 4 kb, in most cases even longer targets can be successfully amplified.

Red PCR Master Mix and Red Hot Start PCR Master Mix contain an inert red dye which allows identification of the reactions which contain enzyme. The dye has no adverse effect on PCR.

Green PCR Master Mix and Green Hot Start PCR Master Mix contain two dyes (blue and yellow) that separate during electrophoresis to monitor migration progress. Reactions assembled with Green PCR Master Mix have sufficient density for direct loading onto agarose gels. Green Master Mix is recommended for any amplification reaction that will be visualized by agarose gel electrophoresis and ethidium bromide staining. The dyes absorb between 225–300nm, making standard A260 readings to determine DNA concentration unreliable.