Low-Tox-H Rabbit Complement for Sensitive CDC Assays

Optimised Complement for HLA-DR Tissue Typing and Lymphocytotoxicity Assays 

Cedarlane’s Low-Tox®-H Rabbit Complement is a specially processed rabbit serum designed to deliver high complement activity with reduced cytotoxicity, making it ideal for use in HLA-DR tissue typing and standard lymphocytotoxicity assays involving human B-lymphocytes. 

With over 50 years of expertise in complement manufacturing, Cedarlane is a global leader in providing reagent complement solutions for clinical and research applications. The Low-Tox®-H variant has been specifically refined to minimise background toxicity, ensuring consistent and reliable results—particularly with sensitive peripheral blood B-cell populations. 

Key Features and Benefits 

• Low background toxicity to human B-lymphocytes 

• High-performance complement activity 

• Specifically designed for HLA-DR tissue typing 

• Suitable for standard microlymphocytotoxicity assays 

• Processed from normal rabbit serum using proprietary low-toxicity methods 

• Compatible with assays outlined in the NIAID Manual of Tissue Typing Techniques 

Applications 

• HLA-DR tissue typing 

• Lymphocytotoxicity assays 

• Immunogenetics labs and histocompatibility testing 

• Ideal for systems where standard rabbit serum exhibits excessive cell lysis 

In typical use, cells are first incubated with the relevant antiserum, then exposed to Low-Tox®-H Rabbit Complement. After incubation, cytotoxicity is scored according to the specific assay method. Complement controls are essential to account for system-specific background effects. 

🔍 Product Formats and Customisation 

Cedarlane offers Low-Tox®-H Complement in a variety of sizes and formats, with options for: 

• Bulk quantities 

• Reserved lot evaluations 

• Custom formulations