Tissue: Mouse Colon and Brain Prepared Microscope Slides

Tissue Slides for Fluorescent Microscope Setup, Calibration, and Performance Validation 

OptiSlides Tissue Slides are high-performance fluorescent reference slides designed to support microscope setup, troubleshooting, and system validation across a wide range of light microscopy platforms. Developed by scientists, for scientists, these tissue slides combine rich biological complexity with crisp, multi-channel fluorescence for accurate performance assessment and demonstration of imaging capabilities. 

Designed for Real-World Imaging Performance 

Our standard tissue slide features mouse colon, available in 4-colour, 4-channel and 6-channel configurations (including Near Infrared fluorophores for 735–785 nm laser lines). The sample is stained to highlight: 

• DAPI (nuclei, blue) 

• PGP9.5 (neurons, green) 

• White blood cells (magenta) 

• Blood vessels (cyan) 

The resulting image, shown here as a maximum intensity projection from a laser scanning confocal microscope (10x objective), captures biologically relevant structures in stunning detail. 

6-Channel Near Infrared (NIR) Variants Available 

OptiSlides are also available in 6-channel configurations incorporating Near Infrared (NIR) fluorophores, extending performance validation into longer wavelengths for advanced multiplex imaging and spectral separation. 

Mouse Colon 6-Channel NIR 

Channels and Targets Include: 

Mouse Brain 6-Channel NIR 

Channels and Targets Include: 

These variants are designed to support: 

• Chromatic integrity assessment across visible and NIR channels 

• Depth penetration validation 

• Accurate image registration in X, Y, and Z 

• Multi-channel alignment and bleed-through evaluation 

Validated for use on NIR-enabled fluorescence microscopy systems, including confocal and widefield platforms. 

Microscopy Applications 

OptiSlides Tissue Slides are compatible with nearly all major light microscopy platforms, including: 

• Laser Scanning Confocal 

• Spinning Disk Confocal 

• Multiphoton 

• Widefield and Brightfield 

• STED Super-Resolution 

• Darkfield and Polarised Light Systems 

These slides are ideal for: 

• Validating filter sets and camera settings 

• Assessing chromatic fidelity and image registration (X, Y, Z planes) 

• Demonstrating multi-channel and spectral imaging capabilities 

Tissue Thickness Options 

• Standard thicknesses: 100 μm and 200 μm 

• Ultra-thick samples: Up to 400 μm (available in 2-colour format by request) 

• Mounted in VectaShield Plus liquid medium using double-sided adhesive spacers 

• Covered with a #1.5 coverslip, sealed with polish or sealant 

These thick tissue sections provide an excellent model for testing optical sectioning, depth penetration, and Z-stack acquisition capabilities on advanced imaging systems. 

Reusable and Easy to Maintain 

• Gently blot immersion oil using Kimwipes® or lens paper 

• Clean with 70% ethanol using a cotton-tipped applicator 

• Takes just a minute, leaving the coverslip clean and ready for re-imaging 

 Enquire below to request a quote or more information.